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Chargaff's 2nd rule seems to generally be the consequence of a far more intricate parity rule: in just a single strand of DNA any oligonucleotide is existing in equal numbers to its reverse complementary nucleotide. Due to computational specifications this hasn't been verified in all genomes for all oligonucleotides. It's been verified for triplet oligonucleotides for a significant knowledge set.
The 1st rule retains that a double-stranded DNA molecule globally has share base pair equality: %A = %T and %G = %C. The demanding validation of your rule constitutes the basis of Watson-Crick pairs inside read this the DNA double helix model